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Pulse Oximetry Lab
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Highlights: This lab allows students to explore spectrophotometers and the functioning of pulse oximeters.
Abstract: The simulated pulse-ox device uses bromothymol blue, a common pH indicator, as a blood analog. Simply breathing on a solution of bromothymol blue will introduce carbon dioxide from your breath into the solution. The CO2 reacts with the solution to form carbonic acid which increases the acidity of the solution. As the pH of the solution changes, the color of the solution also changes. In this lab, students examine the change both visually and with a spectrophotometer linking observations to the spectral characteristics of deoxygenated and oxygenated blood as measured by a pulse-oximeter.
Resource Types: Lab, Instructor supplement, Project, Exam problem
Authors:

Justin Dunlap

Portland State University

Ellynne Kutschera

Portland State University

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Pulse Oximetry Lab

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Copyright:   2021 Portland State University

License:   CC0 – Public Domain. No restrictions.

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